Figure 6

Toxic effect of mutant huntingtin expression in differentiated cells. A: Quantification of neurite extension as a function of cell number using InCell Western determination of tubulin versus nuclear ratios in differentiated HN10 lines one and two days after induction of wild-type (black bars) and mutant (white bars) Htt-exon1 with 500 nM RSL1. B, C: Total cell protein determinations in the presence or absence of 72Q Htt-exon1 expression in proliferating (B) or differentiated (C) HN10 cells. All graphs: n = 3, * p < 0.05, ** p < 0.01.