Figure 1

ASGFP localizes to the presynaptic terminal by 17DIV. Primary rat E18 hippocampal neurons were transfected with either ASGFP (top and middle rows, green) or eGFP (bottom row, green). After 17 days in vitro (DIV), cells were fixed and immunostained for MAP2 (top row, red), or synapsin I (middle and bottom rows, red), and visualized for immunofluorescence. Scale bar = 10 μm.