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Figure 4 | Molecular Neurodegeneration

Figure 4

From: Posttranslational modification and mutation of histidine 50 trigger alpha synuclein aggregation and toxicity

Figure 4

SDGC analysis of recombinant WT, H50Q, and H50R aSyn. Recombinant human WT aSyn is clearly detected in the low density factions from 16 to 22 (row 1 of the upper panel). Oligomerized aSyn is characterized by a signal shift towards higher density fractions, which is exemplarily displayed by HNE-aSyn and n-aSyn consisting of oligomeric aSyn (rows 1 and 2 of the lower panel). Large fibrillar aggregates appear in the first exclusion fractions (row 3 of the lower panel). H50 mutations (H50Q and H50R) increase aSyn immunosignal in the 60% high density exclusion fraction (arrows, rows 2 and 3 of the upper panel).

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Molecular Neurodegeneration

ISSN: 1750-1326