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Fig. 3 | Molecular Neurodegeneration

Fig. 3

From: Single molecule array measures of LRRK2 kinase activity in serum link Parkinson’s disease severity to peripheral inflammation

Fig. 3

Serum LRRK2 and pT73-Rab10 proteins originate primarily from bone marrow immune cells. (A) Representative cytographs and column graphs showing the analysis of live (7AAD- 7-Aminoactinomycin D negative) leukocytes in blood from outbred male CD-1 mice before and six-days after 11 grays of radiation. (B) SiMOA analysis of serum from these mice for (B) total LRRK2, (C) total Rab10 and (D) the ratio of pT73-Rab10 to total Rab10. (E) Cytographs show the analysis of host CD45.2 and donor CD45.1 mice before and after radiation, and the column graph shows the mean of the ratio of CD45.1 cells to total CD45 cells (n = 3 male mice). Bone marrow transplantation of donor Tg-WT-Lrrk2 cells into host Lrrk2−/− mice restores levels of (F) total LRRK2, (G) total Rab10 and (H) the ratio of pT73-Rab10 to total Rab10. Each dot shows mean values from the analysis of a single animal (all ~ 2 months of age), and p values are calculated from paired (before and after) sample t-tests

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Molecular Neurodegeneration

ISSN: 1750-1326