Fig. 5

UA restores upcycling of damaged lysosomes via lysophagy. A Diagram depicting the basis of tfGal3 lysophagy tandem fluorescent reporter assay. B Representative images of ARPE-19 cells expressing the tfGal3 reporter, 1 mM LLOMe was used as positive control. Permeabilized lysosomes (RFP⁺GFP⁺, gray) and lysophagic lysosomes (RFP⁺GFP⁻, magenta) can be observed, nuclei were counterstained with DAPI (blue). C Quantification of the number of damaged lysosomes and lysophagic lysosomes as shown in B. Lysophagy efficiency is reported as the number of lysophagy events per permeabilized lysosome, indicative of lysophagic flux. Scale bar, 25 μm. All data are expressed as the mean ± s.e.m. Dots represent biological replicates from three independent experiments. P values were calculated using 1-way ANOVA with Fisher’s LSD post-hoc test. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, ns: not significant