Fig. 2

Cross-sectional association of NULISAseq targets with amyloid pathology (A). A Volcano plot of -log10 (p-value) versus log2(fold change) comparing biomarker abundances (NPQ) in samples from A + participants (n = 49) vs. A- controls (n = 127). Significant targets are shown in red (higher in A +) or blue (lower in A +) circles. Grey circles represent non-significant targets. NPQ (NULISA Protein Quantification) represents the log2-transformation of normalized target counts. B Boxplot distributions of significant NULISAseq targets, separated by A status and visit. P-values on top of the boxplots were for the whole data combining both visits and were determined using linear mixed models (random intercepts) with NPQs as the dependent variable, visit-specific A status as the independent variables, adjusting for covariates age, sex, and APOE ε4 carrier status. Significance determination was based on p-value < 0.005, corresponding to ~ 8% FDR