Fig. 1
From: Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMB
Characterisation of hiPSC-derived microglia cultures. A Schematic showing directed differentiation of hiPSCs to microglia and macrophages (termed hiPSC-derived macrophages and microglia hereafter). B Principal component analysis (PCA) of variance stabilised counts, plotted by their coordinates along the first two principal components of hiPSC microglia (current study denoted Clarke) as well as other publicly available hiPSC microglia [41, 42], other hiPSC derived CNS cell types [36, 43], primary microglia from fetal and adult human brain samples [41], and other primary CNS cell types from human brain samples [44]. C Heatmap showing RNA sequencing normalised gene expression counts of distinct CNS cell types across our healthy control and VCP mutant hiPSC-derived microglia. D Heatmap showing microglial protein marker intensities detected by mass spectrometry across our healthy control and VCP mutant hiPSC-derived microglia. Immunofluorescence images and quantification of (E) IBA1, F P2RY12 and (G) TMEM119. Scale bar: 20 μm. Wilcoxon-test was used to determine significance (** P < 0.01, * P < 0.05)