Fig. 2
From: Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMB
VCP mutant microglia display transcriptomic and proteomic perturbations. A Volcano plot showing log2 fold change in VCP mutant versus healthy control microglia differential gene expression. Genes with significantly (FDR < 0.05) increased expression are shown in red, and those decreased in expression are shown in blue. Data are from 3–4 lines per condition from 1 differentiation. B Gene Ontology (GO) terms enriched in upregulated (red) and downregulated (blue) differentially expressed genes in VCP mutant microglia. C Gene set enrichment analysis for lysosomal lumen genes in VCP mutant microglia. D Volcano plot showing log2 fold change in VCP mutant versus healthy control microglia differential protein abundance from mass spectrometry. Data are from 5 lines per condition from 1 differentiation. E GO terms enriched in upregulated (red) and downregulated (blue) differentially expressed proteins in VCP mutant microglia. F Correlation of VCP vs CTRL microglia in RNAseq (x-axis) with mass spectrometry (y-axis)