Fig. 4
From: Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMB
VCP mutant microglia show partial overlap with LPS-stimulated healthy control microglia. A Schematic showing treatment of healthy control and VCP mutant microglia with LPS and downstream RNA-seq and mass spectrometry analysis and comparison. B Scatterplot showing differential gene expression z-statistical (Wald test) between VCP mutant (x-axis) and LPS related (y-axis) changes. Data are from 3–4 lines per condition from 1 differentiation. C Venn diagram depicting the number of overlapping differentially expressed genes (FDR < 0.05) between VCP mutant and LPS-stimulated microglia. D Scatterplot showing differential protein expression z-statistical (Wald test) between VCP mutant (x-axis) and LPS related (y-axis) changes. Data are from 5 lines per condition from 1 differentiation. E Venn diagram depicting the number of overlapping differentially expressed proteins (FDR < 0.05) between VCP mutant and LPS-stimulated microglia. F Volcano plot of log2 fold change in differential gene expression between LPS-stimulated VCP mutant versus LPS-stimulated healthy control microglia. G, H PROGENy signaling pathway activity normalized enrichment scores (y-axis) in LPS-stimulated VCP mutant versus LPS-stimulated healthy control microglia. I DoRothEA transcription factor regulon analysis in VCP mutant microglia. J Heatmap showing differentially secreted cytokines and chemokines in LPS-stimulated VCP mutant versus LPS-stimulated healthy control microglia. K Median fluorescence intensity (MFI) plot for IL-1B release in LPS-stimulated VCP mutant versus LPS-stimulated healthy control microglia