Fig. 6
From: Human VCP mutant ALS/FTD microglia display immune and lysosomal phenotypes independently of GPNMB
GPNMB knockdown reduces inflammatory signalling in VCP mutant microglia. A Western blot for GPNMB and GAPDH loading control in microglia treated with scrambled or GPNMB targeting siRNA. B Quantification of western blot for GPNMB in microglia treated with scrambled or GPNMB targeting siRNA. C Volcano plot of log2 fold change in differential gene expression between VCP mutant microglia treated with GPNMB siRNA or scrambled siRNA. D PROGENy signaling pathway activity normalized enrichment scores (y-axis) in GPNMB siRNA treated VCP mutant microglia. E DoRothEA transcription factor regulon analysis in GPNMB siRNA treated VCP mutant microglia. Data are from 3 lines per condition from 1 differentiation. Quantification of (F) CXCL10 and (G) IL-6 secretion from healthy control and VCP mutant microglia treated with scrambled or GPNMB siRNA. Statistics are from a generalised linear model accounting for experimental repeats. Data are from 4 lines per condition from 2 differentiations