Fig. 7

VCP mutant and LPS-stimulated microglia conditioned media activates JAK-STAT signalling in motor neurons and astrocytes. A Schematic of microglia conditioned media transfer in unstimulated and LPS-stimulated microglia. B Volcano plot of log₂ fold change in differential gene expression between VCP mutant microglia conditioned media and healthy control microglia conditioned media on hiPSC derived healthy control motor neurons and astrocytes. C PROGENy signaling pathway activity normalized enrichment scores (y-axis) in unstimulated VCP mutant microglia conditioned treated healthy control neurons and astrocytes. D DoRothEA transcription factor regulon analysis in unstimulated VCP mutant microglia conditioned treated healthy control neurons and astrocytes. E Volcano plot of log₂ fold change in differential gene expression between LPS-stimulated VCP mutant microglia conditioned media vs LPS-stimulated healthy control microglia conditioned media treated healthy control neurons and astrocytes. F PROGENy signaling pathway activity normalized enrichment scores (y-axis) in LPS-stimulated VCP mutant microglia conditioned media vs LPS-stimulated healthy control microglia conditioned media treated healthy control neurons and astrocytes. G DoRothEA transcription factor regulon analysis in LPS-stimulated VCP mutant microglia conditioned media vs LPS-stimulated healthy control microglia conditioned media treated healthy control neurons and astrocytes. Data are from 3 healthy control lines per condition from 1 differentiation for both motor neurons and astrocytes