Fig. 3
From: VCP regulates early tau seed amplification via specific cofactors
Acute exposure of inhibitors differentially impacts tau aggregation. A Timeline of experiment: 1 h exposure of tau biosensor cells to inhibitors, followed by 4 h of 25 nM tau before washout. B ML-240 increased tau seeding. P values: ns = 0.4353, **** < 0.0001 (C) NMS-873 dose-dependently decreased tau seeding. P values: ** 0.0030, **** < 0.0001 (D) Proteasome inhibitor, MG132, increased tau seeding. P values: ns = 0.8492, * 0.0415, ** 0.0024, **** < 0.0001. Error bars represent S.D. Representative data of n = 3 independent experiments, with each data point derived from technical triplicate. E Fluorescence microscopy confirmed the effects of VCP and proteasome inhibition on tau seeding. Scale bar = 50 μm. F Compound treatment did not change tau-AF-647 uptake as measured by flow cytometry. Error bars represent S.E.M (n = 3). P values: ns = 0.9960, 0.9992, 0.1742, in order of bars on the graph. One-Way ANOVA with a 95% confidence interval