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Fig. 7 | Molecular Neurodegeneration

Fig. 7

From: Distinct regulation of Tau Monomer and aggregate uptake and intracellular accumulation in human neurons

Fig. 7

siRNA-mediated downregulation of some molecular mediators differentially impacts the intracellular accumulation of Tau Monomers and small fibrils. A Timeline of the experimental scheme. iPSC-derived neuronal progenitor cells or LUHMES cells were seeded in 96 well-plates in the differentiation medium. Cells were treated with 10nM siRNA at day 2 of differentiation. iPSCNSs and LUHMES neurons were treated with fluorescently labeled Tau at days 10 to 12 and 6 to 8, respectively. Fluorescence measurements were implemented after 16 to 24 h of treatment. Intracellular accumulation of labeled Tau Monomers and small fibrils were shown in B, C,D iPSCNs, and E, F,G LUHMES neurons that were treated with siRNAs of LRP1, EXT2, and VPS35. Error bars represent SEM; n = 9–18. One-way ANOVA ***p < 0.001, ****p < 0.0001. Error bars represent SEM. NC: negative control siRNA

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Molecular Neurodegeneration

ISSN: 1750-1326