Fig. 1
From: SIRT2 and ALDH1A1 as critical enzymes for astrocytic GABA production in Alzheimer’s disease
Sirt2 is highly expressed in cortical and hippocampal astrocytes. A Schematic diagram of the putrescine-to-GABA conversion pathways with predicted candidate enzymes. B Experimental timeline for immunocyctochemistry and Next Generation RNASeq. C Bar graph of FPKM values of sirtuin family members (top) and histone deacetylases (bottom) in primary cortical and hippocampal astrocyte cultures (individual data points refer to cell culture batches. D Immunostaining for SIRT2 and GFAP in primary astrocyte cultures (Scale bar 40 μm). E Differential expression analysis of genes using RNASeq in astrocytes upon Aβ treatment (Values above bars indicate FDR step-up from DeSeq2 differential analysis). F Top Left, pie chart representation of ratio of Sirt2 transcriptional variants in astrocytes; Top Right, bar graph of differential expression analysis of Sirt2 transcriptional variants (Values above bars indicate p-value from DeSeq2 differential analysis); Bottom, representation