Fig. 2
From: SIRT2 and ALDH1A1 as critical enzymes for astrocytic GABA production in Alzheimer’s disease
SIRT2 and ALDH1A1 are involved in the conversion of putrescine to GABA in astrocytes (See also Fig. S1). A and D Experimental timeline for GABA staining and quantification with pharmacological (A) and genetic (D) inhibition. B and E Pseudo-colored representative images from immunostaining for GFAP and GABA in primary cultured astrocytes treated with putrescine in the presence or absence of EX527 or AGK2 (B) or putrescine-treated primary cultured astrocytes expressing Scr/shALDH1A1/shSIRT2-mCherry (E). C and F Truncated violin plot for GABA intensity in GFAP-positive cells from (B), and mCherry-positive (shRNA-expressing) cells from (E). Data represents Median ± quartiles. Values above graphs represent p-value from indicated statistical test (Ordinary one-way ANOVA)