Fig. 5
From: SIRT2 and ALDH1A1 as critical enzymes for astrocytic GABA production in Alzheimer’s disease
SIRT2 and ALDH1A1 are involved in GABA production in vivo. A Schematic of virus injection and experimental timeline for tonic GABA recordings in B6 mice (Left); Virus constructs used for genetic knockdown of target enzymes (Middle); Representative DIC and fluorescence images of virus infection and spread (Right). B Representative traces of tonic GABA current recorded in the DG neurons from putrescine-incubated brain slices of AAV-GFAP-Cre + Lenti-pSico-Scr/shSIRT2/shALDH1A1-GFP-injected B6 mice. C Representative bar graphs for tonic GABA current (Left), spontaneous IPSC amplitude (Middle) and frequency (Right) from recorded traces. Data represents Mean ± SEM. Data points represent individual cells recorded from N = 5 in each group. Values above graphs represent p-value from corresponding statistical test (Ordinary One-way ANOVA, Tukey’s multiple comparisons)