Fig. 6

SIRT2 and ALDH1A1 are involved in Alzheimer’s Disease pathology (See also Fig. S2). A Images representing ALDH1A1 and GFAP immunoreactivity in the CA1 stratum radiatum in the hippocampus of APP/PS1 mice (Scale bar 20 μm). B Violin plot representing the astrocytic ALDH1A1 intensity (Left) and GFAP immunoreactivity (Right) in APP/PS1 mouse hippocampus (N = 3, Mann–Whitney test). C Lattice SIM images of SIRT2 immunostaining and localization with GFAP immunoreactivity near amyloid beta plaques (Stained with PyrPeg, white) in APP/PS1 mice (Scale bar 10 μm). D Violin plot representing SIRT2 intensity in GFAP-positive astrocytes in APP/PS1 mice (N = 3, Mann–Whitney test). E Dot-plot representation of Pearson’s coefficient for colocalization of SIRT2 and GFAP in Lattice SIM images from (C) (individual data point represents one image, N = 3; Unpaired t-test). F Schematic representation of animal experimental timeline (Left), virus constructs for cell type-specific genetic knockdown (Middle) and representative DIC and fluorescence images showing virus expression and spread (Right). G Y-maze schematic diagram (Top) and bar graph representing spontaneous alternation (in percentage) of APP/PS1 mice in Y-maze behavior task (Bottom; individual data point represents one animal; Ordinary one-way ANOVA). H Representative traces of tonic GABA current recorded from DG granule layer neurons of APP/PS1 mice from (G). I Bar graphs representing the tonic GABA current (Left), spontaneous IPSC amplitude (Middle) and frequency (Right) from recorded traces (individual data point represents one cell recorded, N = 4–6 each group; Ordinary one-way ANOVA). Data represents Median ± quartiles or Mean ± SEM. Values above graphs refer to p values from statistical analyses