Fig. 3

Microglial CD2AP haploinsufficiency attenuates microglia activation in 5xFAD mice. A, B Venn diagram analysis revealed that the expressions of 29 downregulated genes (A) and 55 upregulated genes (B) in 5xFAD mice compared to controls were reversed when microglial CD2AP was deficient. C Important Gene Ontology terms for Biological Process (GO BP) based on the 29 genes from (A, in pink) and the 55 genes from (B, in green). D Important Gene Ontology terms for Cellular Component (GO CC) based on the 55 genes from (B). E Gene expression heatmap of selected microglia-related genes identified in the 55 genes from (B). F Representative images showing co-immunostaining of Iba1 (magenta), Dectin1 (white), and 6E10 (green) in the hippocampus of 7-month-old 5xFAD;Cd2ap^fl/+ and 5xFAD;Cd2ap^fl/+;Cx3cr1-cre mice. Scale bars: 20 μm. The percentages of Dectin1⁺ microglia in total microglia were compared. n = 5 mice per genotype. Unpaired Student’s t test. Data are presented as mean ± SEM. *P < 0.05