Fig. 5

ApoeCh has minimal impact on phosphorylated tau accumulation. a Representative 10 × image of hemispheric coronal brain image of a PS19 mouse immunolabeled for phosphorylated tau using AT8 (green) highlighting brain regions with high pathological manifestation, dentate gyrus (DG) and piriform cortex (PIRI). Scale bar = 500 µm b Representative 20 × confocal images of dentate gyrus (top) and piriform cortex (bottom) from 9-mo old PS19 and PS19;ApoeCh mice immunolabeled for AT8 (green). Scale bar = 100 µm. c-d Percentage of AT8⁺ area coverage per FOVs of 9 mo-old WT, ApoeCh, PS19, and PS19;ApoeCh mice in the DG (c) and PIRI (d). e-j Phosphorylated tau T231 (e-g) and total tau (h-j) measured via MSD in RAB, RIPA, and formic acid fraction of the micro-dissected cortices of 9-mo-old WT, ApoeCh, PS19, and PS19;ApoeCh mice. k Western blot images of HT7-detected total tau and pThr231 tau in cortical RIPA-soluble fraction of 9-mo PS19 and PS19;ApoeCh mice. l, m HT7 (l) and pThr231 signal (m) normalized to total protein stain (LPS). n Western blot images of total tau detected with Dako antibody and p-tau by AT8 in cortical RIPA-soluble fraction of 9-mo PS19 and PS19;ApoeCh mice. o, p Dako (o) and AT8 (p) signal normalized to TPS. n = 3–6 mice/sex/genotype. In panels with graphs, sex of individual animals is denoted by pink (female) or blue (male) circles. Data are represented as mean ± SEM. Student’s t-test, unpaired. Two-way ANOVA followed by Tukey’s post hoc tests to examine biologically relevant interactions. Statistical significance is denoted by *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001