Fig. 6

Reduced microglial and astrocytic response to tau induced by ApoeCh. a, c Representative confocal images of immunostained dentate gyrus for GFAP (blue, a) and IBA1 (red, c) of 9-mo-old WT, ApoeCh, PS19, and PS19;ApoeCh mice. Scale bar = 100 µm. b,d Quantification of total volume of GFAP⁺ cells (b) and IBA1⁺ cells (d), n = 4–6 mice/sex/genotype. Sex of individual animals is denoted by pink (female) or blue (male) circles. Data are represented as mean ± SEM. Two-way ANOVA followed by Tukey’s post hoc tests to examine biologically relevant interactions. Statistical significance is denoted by *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. e Cell types plotted in XY space. f Proportion of the number of cells in each major cell type, grouped by genotype. g-k Immunofluorescence images for Human Tau, Phospho-Tau (S214), CD11c, IBA1, and GFAP in representative brains of WT, ApoeCh, PS19, and PS19;ApoeCh mice. l Aggregate expression of differentially expressed proteins (DEPs) in microglia, astrocytes, and neurons across the four genotypes. Expression is normalized for the number of brains in each genotype