Fig. 6

C1q minimally competes with apoE4 and oAβ42 for binding to TREM2. (A&B) Schematic of competition binding BLI experiments. (C&D) BLI sensorgrams for (C) apoE4 competing C1q binding to TREM2 and (D) C1q competing apoE4 binding to TREM2. Red sensorgrams are TREM2 binding to (C) 1000 nM C1q or (D) 500 nM apoE4 alone while blue sensorgrams show competition experiments where (C) 500 nM apoE4 or (D) 1000 nM C1q are bound first. (E&F) BLI binding magnitudes for TREM2 binding to (E) 1000 nM C1q alone or when pre-binding 500 nM apoE4 or (F) 500 nM apoE4 alone or when pre-binding 1000 nM C1q. Percent decrease in Association 2 binding signal in the presence of the competitor is shown above the bars. (G & H) Schematic of competition binding BLI experiments. (I&J) BLI sensorgrams for (I) oAβ42 competing C1q binding to TREM2 and (J) C1q competing oAβ42 binding to TREM2. Red sensorgrams are TREM2 binding to (I) 1000 nM C1q or (J) 500 nM oAβ42 alone while blue sensorgrams show competition experiments where (I) 500 nM oAβ42 or (J) 1000 nM C1q are bound first. (K&L) BLI binding magnitudes for TREM2 binding to (K) 1000 nM C1q alone or when pre-binding 500 nM oAβ42 or (L) 500 nM oAβ42 alone or when pre-binding 1000 nM C1q. Percent decrease in Association 2 binding signal in the presence of the competitor is shown above the bars