Fig. 9

C1q and apoE4 compete IL-34 for binding to TREM2, but oAβ42 does not compete IL-34 for binding to TREM2. (A&B) Schematic of competition binding BLI experiments. (C&D) BLI sensorgrams for (C) apoE4 competing IL-34 binding to TREM2 and (D) IL-34 competing apoE4 binding to TREM2. Red sensorgrams are TREM2 binding to (C) 500 nM IL-34 or (D) 500 nM apoE4 alone while blue sensorgrams show competition experiments where (C) 500 nM apoE4 or (D) 500 nM IL-34 are bound first. (E&F) BLI binding magnitudes for TREM2 binding to (E) 500 nM IL-34 alone or when pre-binding 500 nM apoE4 or (F) 500 nM apoE4 alone or when pre-binding 500 nM IL-34. Percent decrease in Association 2 binding signal in the presence of the competitor is shown above the bars. (G & H) Schematic of competition binding BLI experiments. (I&J) BLI sensorgrams for (I) IL-34 competing oAβ42 binding to TREM2 and (J) oAβ42 competing IL-34 binding to TREM2. Red sensorgrams are TREM2 binding to (I) 500 nM IL-34 or (J) 500 nM oAβ42 alone while blue sensorgrams show competition experiments where (I) 500 nM oAβ42 or (J) 500 nM IL-34 are bound first. (K&L) BLI binding magnitudes for TREM2 binding to (K) 500 nM IL-34 alone or when pre-binding 500 nM oAβ42 or (L) 500 nM oAβ42 alone or when pre-binding 500 nM IL-34. Percent decrease in Association 2 binding signal in the presence of the competitor is shown above the bars. (M&N) Schematic of competition binding BLI experiments. (O&P) BLI sensorgrams for (O) IL-34 competing C1q binding to TREM2 and (P) C1q competing IL-34 binding to TREM2. Red sensorgrams are TREM2 binding to (O) 1000 nM C1q or (P) 500 nM IL-34 alone while blue sensorgrams show competition experiments where (O) 500 nM IL-34 or (P) 1000 nM C1q are bound first. (Q&R) BLI binding magnitudes for TREM2 binding to (Q) 1000 nM C1q alone or when pre-binding 500 nM IL-34 or (R) 500 nM IL-34 alone or when pre-binding 1000 nM C1q. Percent decrease in Association 2 binding signal in the presence of the competitor is shown above the bars