Fig. 3

Knockdown of astrocytic HDAC7 attenuates cognitive impairments in PS19 mice. A-F Spatial memory was evaluated using Morris Water Maze (MWM) test at 30 days post-AAV injection. A Escape latency to the targeted platform during the training trial. B Representative traces of the mice traveled in the in the probe trial after removing the platform. C First latency to reach the platform region, D exploring time in the targeted zone, (E) target crossings and F swimming speed in the probe trial. G Discrimination index of WT and PS19 mice in the probe trial of Novel Object Recognition (NOR) test. For behavioral tests, n = 11 (WT-Vec), 10 (WT-shHD7), 15 (PS19-Vec), 15 (PS19-shHD7) mice per group. H Representative images showing neuronal dendrites and spines by Golgi staining in the hippocampus of WT and PS19 mice after AAV injection. Scale bar: 10 μm. I Sholl analysis of dendritic intersections. n = 28 (WT-Vec), 32 (WT-shHD7), 31 (PS19-Vec), 29 (PS19-shHD7) from 3 mice per group. J Quantification of spine numbers, n = 33 (WT-Vec), 32 (WT-shHD7), 54 (PS19-Vec), 43 (PS19-shHD7) from 3 mice per group. Statistical significance was determined by two-way ANOVA (A) or one-way ANOVA (C-G and J) or with Tukey’s post hoc analysis. Data are shown as mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, n. s, not significant