Fig. 1
From: Srebf2 mediates successful optic nerve axon regeneration via the mevalonate synthesis pathway
LCM-seq analysis of 3 dpi retinal ganglion cell layer (GCL) identifies upregulation of the cholesterol synthesis pathway. (A) Representative image from pre- and post-GCL LCM retina section. (B) Volcano plot (|FoldChange| > 1, FDR < 0.05) of differentially expressed gene from 3 dpi (n = 3) vs. uninjured GCL (n = 2). (C) GSEA of DEGs genes based on zebrafish WikiPathways. (D) Heatmap of cholesterol-related transcriptional regulators, cholesterol synthases, and cholesterol trafficking genes of 3 dpi vs. uninjured from GCL. Gene names in red or blue indicates p < 0.05 by DEseq2 in 3 dpi group versus control where red represents upregulated genes and blue represents downregulated genes