Fig. 5

Conditional expression of constitutively active srebf2 rescues loss-of-function but does not accelerate regeneration. (A) mCherry expression in whole mount Tg(hsp70l: nSrebf2-2AmCherry, cmlc: GFP) zebrafish retina GCL before heat shock (Pre) and 4-hours post heat shock (4 h). (B) Expression of selected mev/chol synthesis pathway genes in post heat shock retina measured by qRT-PCR by one-way ANOVA with Bonferroni post hoc test, n = 4 for each group. (C) Representative images of axon regeneration into the optic tectum after treatment with control MO (C-MO) or srebf2-MO2 with and without heat shock rescue (+ HS) by Tg(hsp70l: nSrebf2-2AmCherry, cmlc: GFP). (D) Quantification of regeneration into the optic tectum. Red boxes represent p < 0.05 in C-MO vs. srebf2-MO2 group at the corresponding rostral to the caudal region; green boxes represent p < 0.05 in C-MO vs. C-MO + HS by two-way ANOVA with Fisher’s LSD post hoc test, no other comparisons were statistically significant, n = 5–6 for each group. (E) Representative images of the DLR on various days post nerve injury with C-MO, srebf2-MO2, and srebf2-MO2 plus transgenic heat shock rescue. (F) Quantification of the DLR in each treatment group over time by two-way ANOVA with Bonferroni post hoc test, n = 5–6 for each group. + HS indicates daily heat shock treatment starting at day 0. Scale bar in A = 50 μm. Scale bar in C = 500 μm and 200 μm. * p < 0.05, ** p < 0.01, *** p < 0.001