Fig. 5

Nuclear material is a core constituent of cortical Lewy bodies. a Example widefield images (i. 20 × objective) cases of dementia with Lewy bodies (DLB) showing Lewy bodies (LBs) as detected via alpha-synuclein phosphorylated at serine 129 (pS129), \(\gamma\) H2AX and b) 53BP-1 immuno-fluorescence, with nuclei are co-stained with DAPI. Digitally zoomed images (ii) show LBs immunoreactivity (arrowhead) and Lewy neurites (arrows) in greater magnification. Note the colocalization of γH2AX with extranuclear pS129 LBs (a) and absence of colocalization of 53BP-1 with LBs (b). c Confocal image of YH2AX and d) H3K27me3 reactive LBs. Quantification of cii) γH2AX and dii) H3K27me3 positive (γH2AX +) and negative (γH2AX -) LBs in examined cases. Data shown as percentage LBs positive and negative for γH2AX and H3K27me3. Scale bar in a.i + b.i = 100 µm, in a.ii + bii = 50 µm and in c + d = 20 µm