Fig. 10

Astrocytic plaques in CBD frontal cortex associate with FTL laden microglia. A FTL-positive cellular structures are associated with AT8 phospho-tau in small (series 1–2, 5) and large (series 3–4, 6) APs. In larger and more mature APs FTL-positive cells are increased in size and become localized in proximity to a center of APs (series 3–4). B Triple labelling of FTL (green), AT8 (magenta), and GFAP (red, series 1) or IBA1 (red, series 2) show that large FTL-positive cells located in the centers of mature APs have microglial, but not astrocytic identity. C Tile image of triple FTL/AT8/IBA1 labeling across CBD cortical tissue showing the frequency and extent of co-localization between FTL laden microglia and phospho-tau APs. D High magnification image of an FTL + microglia cell infiltrating an astrocytic plaque. Grey filled arrowheads denote processes with FTL/AT8/IBA1 triple co-localization. Abbreviations: CBD, corticobasal degeneration; FTL, ferritin light chain; IBA1, microglial marker ionized calcium-binding adapter molecule 1; GFAP, glial fibrillary acidic protein; APs, astrocytic plaques