Fig. 8

VGF co-localizes with AT8 phospho-tau aggregates in AD and PiD human autopsy cases. A In AD cases, VGF was occasionally sequestered into NFTs (series 1–2). B Neuronal PiD inclusions were co-localized with VGF, which associated within the cores of most PBs, ranging from spherical to horseshoe-shaped (series 1–5). Glial inclusions were mostly found not to be associated with VGF (series 6). 60 × tile imaging demonstrates the association of sequestered VGF to grey matter PBs (series 7–9) and variable white matter glial inclusions (series 10–12). C VGF did not co-localize in APs of CBD (series 1), and most oligodendroglial CBs (series 2), or NT pathology (series 3). D PSP TAs (series 1) and CBs (series 2) did not co-localize with VGF. Very rare instances of partial co-localization below the chosen 20% cut-off overlap were observed in a minority of TAs in one PSP case (series 3). E Quantification of VGF -positive phospho-tau lesions from n = 3 cases/tauopathy showing the relative area of overlap between AT8 and VGF in tauopathy lesions. Modified violin plots with data points show individual lesions plotted against relative overlap area. Lesions are categorized into co-localization categories based on their relative area of overlap. Red lines indicate median values. Pie charts underneath the violin plots illustrate the relative proportions of lesions with different degrees of co-localization. Abbreviations: AD, Alzheimer’s disease; PiD, Pick’s disease; CBD, corticobasal degeneration; PSP, progressive supranuclear palsy; NFTs, neurofibrillary tangles; TANCs, tangle-associated neuritic clusters; APs, astrocytic plaques; CBs, coiled bodies; PBs, Pick bodies; GT, glial tau; NTs, neuropil threads; NP, neuritic plaque; SPL, small phospho-tau lesions; TA, tufted astrocytes; GLO-NFT, globose NFTs