Fig. 3

Psoralen ameliorates PD-like motor symptoms and DA neuronal death in the presence of functional NLRP3. Primary neurons were incubated with conditioned medium (MCM) from microglia treated with LPS/ATP and Pso (1 µM). Representative immunostaining (A) and quantification (B) of MAP2 intensity (green). The scale bar represents 20 μm. (C-D) Effects of MCM on the morphology of DA neurons observed by immunohistochemical staining of TH. The scale bar represents 20 μm. (E-G) Travel path, movement distance and speed were recorded in the open field test, n = 6–10. (H) Latency to fall was recorded in the rotarod test, n = 6–8. (I) The time taken to descend a pole (T-TLA) was recorded in the pole test, n = 6–8. (J-K) The levels of dopamine and DOPAC in the striatum homogenate were detected by HPLC, n = 8–10. (L-M) Immunohistochemical staining and counting of TH⁺ neurons in the SNc. The scale bar represents 200 μm. Enlarge vision: 40 μm. (N-O) Staining and counting of Nissl⁺ neurons in the SNc. The scale bar represents 200 μm. Enlarge vision: 40 μm. Data were analyzed by one-way ANOVA, followed by Tukey post-tests. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. ns: no significance