Fig. 4

Psoralen prevents glial NLRP3 inflammasome activation in the MPTP/p mouse model. Immunohistochemical staining and counting of IBA-1⁺ cells (A and C) and GFAP⁺ cells (B and D). The scale bar represents 200 μm. Enlarge vision: 40 μm. (E) Immunofluorescence staining for ASC (green) and microglia marker IBA1 (red) in the SNc of MPTP/p-treated WT and Nlrp3^−/− mice. DAPI stains the nucleus (blue). The scale bar represents 100 μm. (F) Effects of Psoralen (1µM) on NLRP3 inflammasome activation in the LPS/ATP primed-BMDMs from WT and Nlrp3 KO mice. Data were analyzed by one-way ANOVA, followed by Tukey post-tests. ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001. ns: no significance