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Fig. 5 | Molecular Neurodegeneration

Fig. 5

From: Inactivation of NLRP3 inflammasome by dephosphorylation at Serine 658 alleviates glial inflammation in the mouse model of Parkinson’s disease

Fig. 5

Biotinylated-Psoralen directly binds to the NACHT and LRR domains of NLRP3 protein. (A) Docking complex of human NLRP3 protein with Psoralen (Pso). (B) The chemical structure of Biotinylated-Psoralen. (C) Cell lysates of LPS-primed BMDMs were incubated with Pso or Bio-Pso for 2 h, which were then pulled down using streptavidin beads. The NLRP3 inflammasome components in the pull-down (PD) and total (Input) fractions were examined by Western blotting. Flag-tagged NLRP1, AIM2, NLRP3 or NLRC4 (D), NLRP3-LRR, NLPR3-NACHT or NLRP3-PYD (E) was expressed in HEK-293T cells. The HEK-293T cell lysates were incubated with Bio-Pso (10 µM) and then were pulled down using streptavidin beads for Western blotting assay. Bio-Oridonin (1 µM) as a positive drug targeting the NACHT domain of NLRP3 protein

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Molecular Neurodegeneration

ISSN: 1750-1326