Fig. 1
Characterization of CSF1 expression and Rag2-/- effects in hCSF1Bdes mice A. CSF1 expression in brain of homozygous hCSF1Bdes and WT (C57BL/6JRj) mice (mixed sexes, 11 weeks of age) was assessed by western blot analysis with an antibody recognizing both human and rodent CSF1 (left panel), showing equal expression levels. The right panel represent the Ponceau S staining as loading control. Position of molecular weight markers are indicated. B. CSF1 expression in the plasma from homozygous and heterozygous hCSF1Bdes and WT mice measured with ELISA. mean, SD and n = 2–6 are presented on the graph. C. Spleen, thymus and body weight in hCSF1Bdes mice compared to Wild type (WT; C57BL/6j) mice by Mann Whitney U test. Bar plots represent mean ± SD, n = 6–7. D-F. FACS analysis of lymphocytes and NK cells in hCSF1Bdes mice (mixed sexes, 11 weeks of age). Single cells were isolated from the spleen and lymph nodes and stained with anti-CD3, anti-CD19, anti-NK1.1, anti-CD45 and anti-CD11b antibodies, and analyzed by flow cytometry. (D) Representative gating strategies to identify T-, B-, NK T cells and NK cells in spleen (left) and Lymph nodes (right) in WT mice. The histograms represent (E) the distribution and total count of lymphoid cells in spleen, and (F) their distribution in lymph nodes. n = 4–5, bar plots represent mean ± SD. hCSF1Bdes mice are compared to the WT mice by Mann Whitney U test