Fig. 2

Efficient xenotransplantation of human derived microglia. A-C. Representative flow plots (A-B) and quantification of hMG % (C) for hCSF1^Bdes mice xenografted with human microglia, 1 month (A) and 3 months (B) after xenotransplantation. Human microglia (hMG) are represented as hCD45⁺ population after gating for CD11b⁺ cells. Mouse microglia (mMG) are represented as mCD45⁺ population after gating for CD11b⁺ cells. Samples were acquired on BD FACSCanto flow cytometer (A) or Miltenyi Quant Tyto cell sorter (B) and analysed in FlowJo software (C). n = 3–4, bar plot represents mean ± SD. D. Representative images of engrafted human microglia in the brain of hCSF1^Bdes mice at 3 months of age. Microglia cells (both mouse and human) are identified as Iba1.⁺ cells. Xenografted human microglia are identified as hP2RY12 and hNu positive cells. Scalebar, 1mm (top) and 100µm (bottom)