Fig. 3

Human microglial response to Aβ pathology. A-B. Representative flow plots (A) and quantification of hMG % (B) for xenografted App^Hu (panel A left) and App^SAA (panel A, right) mice 6 months after xenotransplantation. Human microglia (hMG) are represented as hCD45⁺ population after gating for CD11b⁺ cells. Mouse microglia (mMG) are represented as mCD45^mid cells after gating for CD11b⁺ cells. Samples were acquired on BD FACS Canto flow cytometer (A) or Miltenyi Quant Tyto cell sorter (B) and analysed in FlowJo software n = 4–6, bar plot represents mean ± SD. C. Representative images of human microglia engrafted in the cortex of App^Hu and App^SAA mice at 6 months of age, labeled with human-specific antibodies for hP2RY12, as well as X34 and 82E1 for Aβ plaques. Scale bar, 100µm. D-E. Single cells have been isolated from mouse brain (half brain hemisphere) (D) and 100µl of blood (E) and analysed by FACS. The graph represents FACS quantification of NK cell number in 6 months old App^Hu and App^SAA xenografted with hMG and in WT mice (C57BL6JRj, not grafted). Bar plot represents mean ± SD (n = 4–6, one-way ANOVA (D), Welch’s ANOVA (E)). F. FACS quantification of % hMG in 6–7-month-old xenografted App^SAA and App^NLGF;hCSF1^Flv mice. n = 6–8, bar plot represents mean ± SD, Mann Whitney U test