Fig. 4
Bulk RNAseq demonstrates the activation of hMG in response to Aβ pathology. A. Vulcano plot of the most differentially up- (red) and down-regulated (blue) genes (Benjamini–Hochberg corrected p-value (padj) < 0.05) when comparing the bulk RNAseq profile of sorted hMG engrafted in AppSAA (n = 5) vs AppHu (n = 5) mice at 6 months of age. B. Vulcano plots highlighting the top 100 marker genes that were significantly differentially expressed (padj < 0.05) in this dataset for disease-associated microglia (DAM), antigen presenting microglia (HLA) and cytokine response microglia (CRM1) and as found in [28]. C. Significantly enriched gene ontology (GO) categories, Uniprot Keywords (UP-KW) and KEGG pathways as found by DAVID. D-F. Immunohistochemistry for hP2RY12 (D), hCD9, hHLA (HLA-DR/DP/DQ) and X34 (E–F) in 6 months old AppSAA vs AppHu mice. D-E,scalebar visible in merge: 200 µm. E. arrow-heads (left panel) pinpoint parenchymal plaque-associated hHLA and hCD9 staining in AppSAA mice, whereas arrows indicate hHLA staining in CNS-associated macrophages. F. Zoom of insert in merge from panel E, scale bar: 50 µm