Fig. 5
From: A stress-dependent TDP-43 SUMOylation program preserves neuronal function
Male TDP-43K408R mice present with age-specific features of ALS. A Representative images and quantification of TDP-43 mislocalization in the lumbar spinal cord of 9-month-old TDP-43K408R male and female mice. Each datapoint represents the average of 4 serial sections, 40 μm apart per individual mouse. Scale bar = 100 μm. (N = 4 per sex/genotype) Unpaired t-test, *** p < 0.0005. See Fig. S12C for sex comparisons. B Representative images at 16 months of age (16MO) and quantification (2MO, 9MO, and 16MO) of neuromuscular junction (NMJ) innervation in the tibialis anterior of male TDP-43K408R mice. > 80 NMJs were quantified per animal. Data presented as mean ± SEM. (N = 3–4 per genotype) 2-Way ANOVA with Tukey’s multiple comparison analysis, *p < 0.05, ****p < 0.0001. C Representative images and quantification of ChAT + motor neurons in the ventral horn of the lumbar spinal cord of male mice. Each datapoint is the average of 4 serial sections spaced 40 μm apart through the lumbar enlargement of the lumbar spinal cord. Unpaired t-test, ** p < 0.005. D Survival curve for male TDP-43K408R mice (Females found in Fig. S7D). Curve comparisons analyzed using Log-Rank test and Gehan-Breslow-Wilcoxon test. *p < 0.05. All assays present Wild Type mice in grey and K408R/K408R mice in purple