Fig. 2

TMEM106B overexpression leads to large TMEM106B + vacuoles in primary neurons. A Numerous vacuoles were observed within the cytoplasm of hTMEM106B(+) neurons, including vacuoles containing fine material and membrane structures (asterisks) as well as empty, electron-clear vacuoles (red arrow heads). Scale bars (5 µm and 2 µm). B EEA1 staining shows normal EEA1 distribution and size and shows no colocalization with enlarged vesicles. C Expansion microscopy of wild-type and hTMEM106B(+) neurons. Scale bar (10 µm and 1000 nm). D The diameter of lysosomes was quantified for 4 cells/genotype. The large vacuolar lysosomes in hTMEM106B(+) neurons have a significantly higher diameter (P < 0.0001) and have fewer small (< 500 nm) lysosomes compared to wild-type neurons (P < 0.0001). Mann–Whitney U test