Fig. 7

Increased TMEM106B levels leads to an anxiety-like phenotype in 12-month-old mice. A Graphical representation of the experimental set-up and composite heatmaps of wild-type (n = 14), hTMEM106B(+) (n = 20), and hTMEM106B(++) animals (n = 8) in the elevated plus maze. Figure created with Biorender. B Quantification of time spent in each zone, C the number of entries in each zone, and D the distance traveled within the maze. hTMEM106B(++) animals spent more time in the closed arms of the maze (P = 0.035) and hTMEM106B(+) animals showed a suggestive decrease in time spent in the closed arms (P = 0.065). hTMEM106B overexpression mice had fewer entries in each of the zones WT vs hTMEM106B(+), P = 0.023; WT vs hTMEM106B(++), P = 0.0044. E Graphical representation of the experimental set-up and composite heatmaps of wild-type, hTMEM106B(+), and hTMEM106B(++) animals in the open field test. Figure created with Biorender. F Quantification of time spent in each zone, G the number of entries in each zone, and H the distance traveled within the maze. hTMEM106B(++) mice spend less time in the corner of the box as opposed to wild-type (P = 0.008) and hTMEM106B(+) (P = 0.006) mice, the composite heatmap shows predominant localization of the hTMEM106B(++) mice to the sides and close to the corner of the box explaining the difference. TMEM106B overexpression animals show an overall decreased number of entries in the different zones of the maze (WT vs hTMEM106B(+), P = 0.025; WT vs hTMEM106B(++), P = 0.026) and travel less distance in each of the zones of the open field box. Together this data suggests that the overexpression animals are, overall, less explorative in the box and maze and show anxiety-like phenotype. Data represented as mean ± SEM. Two-way ANOVA, *, P < 0,05; **, P < 0,01