Fig. 8
TMEM106B overexpression leads to cell loss in the hippocampus of 21-month-old mice. A Representative images of NeuN immunostaining of the hippocampal region of wild-type, hTMEM106B(+), and hTMEM106B(++) mice (n = 4–6/genotype) with zooms for the CA1 and CA3 regions. Scale bars (100 µm). B Quantitative measurements of neuronal loss included the quantification of the NeuN(+) area and quantification of NeuN(+) cells and total cells/mm2 (Hoechst). hTMEM106B(+) show a suggestive reduction in NeuN(+) cells (P = 0.051), a reduction in total cells/mm2 (P = 0.025), and a suggestive decrease in total NeuN(+) area (P = 0.058). hTMEM106B(++) show a reduction in NeuN(+) cells (P = 0.018), a suggestive reduction in total cells/mm.2 (P = 0.056), and a decrease in total NeuN(+) area (P = 0.0035). Data represented as mean ± SEM. ANOVA *, P < 0,05; **, P < 0,01