Fig. 4
‘Endolysosomal’ protein cluster in the TRIAD, EMIF and iNPH cohorts. A Boxplot of ‘Endolysosomal’ Eigenprotein values across controls (CTRL) and Braak stages I-VI in the TRIAD cohort. The protein abundance trend was modelled by a loess-function with the shaded area representing the 95% confidence interval. B Proteins with a correlation coefficient ρ > 0.6 with the cluster’s Eigenprotein value, i.e. proteins belonging to the ‘Endolysosomal’ cluster. C Boxplot of ‘Endolysosomal’ Eigenprotein values across clinical groups, stratified by CSF Aβ status, in the EMIF cohort. D Boxplot of ‘Endolysosomal’ Eigenprotein values across cortical biopsy groups in the iNPH cohort. E Boxplot of ‘Endolysosomal’ Eigenprotein values across Aβ scores, a semiquantitative scale for the Aβ-plaque load in the frontal cortex of patients in the iNPH cohort. A gray-colored point indicates the presence of neurofibrillary tangles in the cortical sample of the respective individual. F Spearman rank-order correlation between the ‘Endolysosomal’ Eigenprotein values and CSF t-tau. G Spearman rank-order correlation between the ‘Endolysosomal’ Eigenprotein values and CSF p-tau181. Eigenprotein values of all plots were z-scored to their respective controls. Statistical significances for all boxplots were assessed using linear models, with age and sex as covariates, followed by post-hoc Tukey’s HSD for pairwise comparisons to control the family-wise error rate. Unadjusted P-values for pairwise comparisons are indicated if relevant. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Abbreviations: CTRL, control; CU/SCI, cognitively unimpaired/subjective cognitive impairment; MCI, mild cognitive impairment; AD, Alzheimer’s disease; A-, Aβ-negative; A + , Aβ-positive