Fig. 2

sEH inhibition attenuated Tau pathology and reactive gliosis in PS19 mice. (A) Representative immunofluorescence staining of hippocampal CA1 region of PS19_Veh and PS19_TPPU mice with AT8 and MC1 antibodies. Scale bar: 100 μm. (B) Quantification of AT8 and MC1 intensities. AT8: PS19_Veh: n = 8♂ and 9♀; PS19_TPPU: n = 10♂ and 11♀. MC1: PS19_Veh: n = 5♂ and 3♀; PS19_TPPU: n = 4♂ and 4♀. (C) Representative Western blots of PHF1- and AT8-positive phospho-Tau and total Tau in hippocampus of 9.5-10-month-old PS19_Veh and PS19_TPPU mice. GAPDH was used as a loading control. (D) Quantification of (C). n = 3♂and 5♀per group. (E) Representative Iba1, COX2 or GFAP immunofluorescence staining images in CA1 area of hippocampus of 9.5-10-month-old mice. Scale bar: 100 µm. (F) Quantification of Iba1, COX2 and GFAP staining intensity. Iba1/GFAP: WT_Veh: n = 7♂and 5♀; WT_TPPU: n = 7♂and 5♀; PS19_Veh: n = 8♂ and 10♀; PS19_TPPU: n = 10♂ and 11♀. COX2: WT_Veh: n = 6♂and 5♀; WT_TPPU: n = 6♂and 5♀; PS19_Veh: n = 4♂ and 6♀; PS19_TPPU: n = 5♂ and 6♀. (G) Representative Iba1 immunofluorescence staining images and 3D skeletonization of microglia in the CA1 area of hippocampus of 9.5-10-month-old mice. Scale bar: 7 µm. (H) Quantification of microglia filament length, surface area, number of branches and volume per cell using the IMARIS software. n = 3♂and 3♀ per group. Filled circle: ♂; open circle: ♀. Data are presented as mean ± SEM. B and D: Student t’s test; F and H: One-way ANOVA with Tukey’s multiple comparison test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0 0.0001. See also Figure S4