Fig. 5

Upregulated neuronal and synaptic pathway genes in TPPU treated PS19 mice. A & B. Volcano plots showing the differentially expressed genes in granule cells of PS19_Veh relative to WT_Veh mice (A) or PS19_TPPU relative to PS19_Veh mice (B). Upregulated genes are highlighted in red color. Downregulated genes are highlighted in blue color. C. GO term pathway enrichment for upregulated genes in granule cells of PS19_TPPU relative to PS19_Veh mice. D. Dot plot showing the expression of glutamatergic synapse and axon guidance related genes that are differentially expressed in PS19_Veh and PS19_TPPU relative to WT_Veh mice (false discovery rate (FDR)-adjusted P < 0.01; absolute value of the log 2 (fold change) (abs. log2 (FC)) > 0.1). Dot color represents row z-scored genotype average of log- and size-normalized gene counts (normalized expression). Dot size represents the percentage of cells in a genotype with positive expression of the gene. E. Western blot analysis of selected pre and postsynaptic proteins in WT_Veh, PS19_Veh and PS19_TPPU hippocampal tissues. GAPDH was used as a loading control. F. Quantification of (E); n = 4♂ and 3♀ per group. Data are presented as mean ± SEM. One-way ANOVA with Tukey’s multiple comparison test. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001