Fig. 6

sEH inhibition enhances synaptic properties in P301S i³Neurons (i³Ns). A. Representative images of triple immunostaining of seven-week-old wild-type (WT) and P301S neurons treated with vehicle or TPPU (2.5 µM) using anti-synapsin1 (SYN1), anti-PSD95, and anti-MAP2 antibodies. Scale bar: 10 μm. The images beneath each main panel are enlarged views of the bracketed areas, marked with arrows indicating Syn1 and PSD95 co-localized synapses over the MAP2⁺ dendrites. Scale bar: 6 μm. B. Quantification of total SYN1, PSD95, and co-localized puncta, as well as the number of respective voxels aligned over 10 μm of MAP2⁺ dendrites; n = 39 per group. Two-way ANOVA. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. Data are presented as mean ± SEM