Fig. 7

TPPU treatment improves synaptic density but not Tau pathology in PFF seeded P301S i³N neurons. A. Representative images of triple immunostaining of seven-week-old PFF seeded P301S neurons treated with vehicle or TPPU (2.5 µM) using anti-synapsin1 (SYN1), anti-PSD95, and anti-MAP2 antibodies Scale bar: 10 μm. The images beneath each main panel are enlarged views of the bracketed areas, marked with arrows indicating Syn1 and PSD95 co-localized synapses over the MAP2⁺ dendrites. Scale bar: 5 μm. (B) Quantification of total SYN1, PSD95, and co-localized puncta, as well as the number of respective voxels aligned over 5 μm of MAP2⁺ dendrites; n = 39 per group. Welch’s test. (C) Representative Western blots of AT8-positive phospho and total Tau in PFF-seeded P301S i³Ns treated with either vehicle or TPPU. GAPDH was used as a loading control. D: Quantification of (C); n = 3 per group. E: Representative MC1 immunofluorescence staining images of PFF-seeded P301S i³Ns treated with either vehicle or TPPU. Scale bar: 50 µm. F: Quantification of percentage of MC1⁺ cells and the surface area of MC1⁺ Tau. P301S + PFF_Veh: 10 images; P301S + PFF_TPPU: 14 images. G: Representative AT8 immunofluorescence staining images of PFF-seeded P301S i³Ns treated with either vehicle. Scale bar: 50 µm. H: Quantification of percentage of AT8⁺ cells and the surface area of AT8⁺ Tau (10 images per group). Data are presented as mean ± SEM. Student’s t test. ***p < 0.001; ****p < 0.0001